Immunocytochemistry/Immunofluorescence analysis of PD-L1 (CD274) in mouse splenocytes using PD-L1 (CD274) Chimeric Monoclonal Antibody (Product # MA5-47932). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with PD-L1 (CD274) Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor488 secondary antibody (1 µg/mL), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom PD-L1 (CD274) Chimeric Monoclonal Antibody, DAPI, merged channels and an isotype control. The isotype control was stained with FITC Chimeric Monoclonal Antibody (Product # MA5-47752) followed by Alexa Fluor488 secondary antibody.